Anti-Collagen VI antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ Collagen VI Antibody (Biotin)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Bovine, Human, Mammal (Other)
Format:	Lyophilized

$546.00each

Catalog Number: LS-B380-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Collagen VI antibody LS-B380 is a biotin-conjugated rabbit polyclonal antibody to Collagen VI from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.
Application: ELISA, IHC, IHC-P, IP, WB
Reactivity: Bov, Hu, Mml
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 1:10000 - 1:50000
Recommended Dilution: IHC-P: 1.25 µg/ml
Recommended Dilution: IP: 1:100
Recommended Dilution: WB: 1:5000 - 1:10000

Target

Target: Collagen VI
Antigen Species: Mammal (Other)
Specificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Epitope: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Antibody

Host Species: Rabbit
Clonality: Polyclonal

Immunogen

Immunogen: Collagen Type VI from human and bovine placenta.

Format

Conjugate/Label: Biotin
Format: Lyophilized
Formulation: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: No
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl