CASP3 / Caspase 3 Antibody
Catalog Number: LS-C12481-200
200 µg / $849.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-Caspase 3 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC-plus™ CASP3 / Caspase 3 Antibody (clone 31A1067)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$383.00each
Catalog Number: LS-B2125-50
Catalog Number: LS-B2125
For bulk orders or custom formulations:
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- Description
- Caspase 3 antibody LS-B2125 is an unconjugated mouse monoclonal antibody to Caspase 3 (CASP3) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
- Application
- IHC, IHC-P, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Apopain | CASP-3 | CASP3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama | caspase-3
- Recommended Dilution: IHC-P
- 5 µg/ml
- Recommended Dilution: WB
- 2 - 3 µg/ml
- UniProt Number
- P42574
- NCBI GenBank
- NM_004346 | NP_004337.2
- SwissProt
- P42574
Target
- Target
- CASP3 / Caspase 3
- Antigen Species
- Human
- Specificity
- Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
- Epitope
- detects both pro Caspase-3 (~32 kD) and the large subunit of the active/cleaved form (~14-21 kD) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this in Western blot applications.
- Gene Name
- CASP3
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 31A1067
- Origin Species
- Human
Immunogen
- Immunogen
- Full length recombinant human Caspase-3 protein.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.05% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
IL-1ß Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene. Thiyagarajan D, Pedersen HL, Seredkina N, Horvei KD, Arranz L, Sonneveld R, Nijenhuis T, van der Vlag J, Rekvig OP. Frontiers in cell and developmental biology. 2018 February;6:7. PubMed: 29468159 PMC: PMC5807897
