CASP3 / Caspase 3 Antibody (N-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$732.00each
Catalog Number: LS-C12476-100
Catalog Number: LS-C12476
For bulk orders or custom formulations:
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- Description
- Caspase 3 antibody LS-C12476 is an unconjugated rabbit polyclonal antibody to Caspase 3 (CASP3) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for Flow, ICC, IF, IHC and WB. Cited in 1 publication.
- Application
- ELISA, FC, ICC, IF, IHC, IHC-F, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Apopain | CASP-3 | CASP3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
- Recommended Dilution: FC
- 1:200
- Recommended Dilution: IHC-F
- 1:200
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P42574
- NCBI GenBank
- NM_004346 | NP_004337.2
- SwissProt
- P42574
Target
- Target
- Human CASP3 / Caspase 3
- Antigen Species
- Human
- Epitope
- Recognizes cleaved human Caspase 3. Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases. Species cross-reactivity: mouse, bovine, and rat.
- Gene Name
- CASP3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic peptide corresponding residues adjacent to Asp175 in N-Terminus of human caspase-3 (KLH coupled).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% Glycerol
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 10 mM HEPES|150 mM NaCl
Overexpression of facioscapulohumeral muscular dystrophy region gene 1 causes primary defects in myogenic stem cells. Xynos A, Neguembor MV, Caccia R, Licastro D, Nonis A, Di Serio C, Stupka E, Gabellini D. Journal of cell science. May;126:2236-45. PubMed: 23525014