Immunofluorescence Microscopy of Mouse anti-IDO1 Antibody. Cells: HEK293 cells. Fixation: 0.5% PFA. Expressing: mouse IDO-1 (left) and mouse IDO-2 (right). Primary antibody: IDO1 (2E2) monoclonal antibody. Antigen retrieval: not required. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO1 as red fluorescent signal with bis-benzimide nuclear counterstain (blue).
IDO1 / IDO Antibody (clone 2E2.6)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745260-25
Catalog Number: LS-C745260
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- Description
- IDO antibody LS-C745260 is an unconjugated mouse monoclonal antibody to mouse IDO (IDO1). Validated for ELISA, Flow, IF, IHC, IP and WB.
- Application
- ELISA, FC, IF, IHC, IP, WB
- Reactivity
- Ms
- Nonreactive Species
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- IDO | Indole 2,3-dioxygenase | Indolamine 2,3 dioxygenase | IDO-1 | IDO1 | INDO | Indoleamine 2,3-dioxygenase | Indoleamine 2,3-dioxygenase 1
- Recommended Dilution: ELISA
- 1:5000 - 1:50000
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1500
- UniProt Number
- P14902
- NCBI GenBank
- AAA36081.1, NM_002164
- SwissProt
- P14902
Target
- Target
- Mouse IDO1 / IDO
- Antigen Species
- Ms
- Gene Name
- IDO1
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 2E2.6
- Isotype
- IgG1
- Origin Species
- Hu
Immunogen
- Immunogen
- IDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.
- Immunogen Type
- Recom
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.95 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl