Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
Immunofluorescence Microscopy of Mouse Anti-IDO1 Antibody. Cells: HEK293 cells. Fixation: 0.5% PFA. Expressing: mouse IDO-1 (left) and mouse IDO-2 (right). Primary antibody: IDO1 (2E2) monoclonal antibody. Antigen retrieval: not required. Secondary antibody: mouse secondary antibody at 1:10000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO1 as red fluorescent signal with bis-benzimide nuclear counterstain (blue).
Monoclonal Mouse anti-Mouse IDO1 / IDO Antibody (clone 2E2.6, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C153837-100
Catalog Number: LS-C153837
For bulk orders or custom formulations:
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- Description
- IDO antibody LS-C153837 is an unconjugated mouse monoclonal antibody to mouse IDO (IDO1). Validated for ELISA, Flow, ICC, IF, IHC and WB. Cited in 1 publication.
- Application
- ELISA, FC, ICC, IF, IHC, WB
- Reactivity
- Ms
- Nonreactive Species
- Human
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- IDO | Indole 2,3-dioxygenase | Indolamine 2,3 dioxygenase | IDO-1 | IDO1 | INDO | Indoleamine 2,3-dioxygenase | Indoleamine 2,3-dioxygenase 1
- Recommended Dilution: ELISA
- 1:5000 - 1:50000
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1500
- UniProt Number
- P14902
- NCBI GenBank
- AAA36081.1 | NM_002164
- SwissProt
- P14902
Target
- Target
- Mouse IDO1 / IDO
- Antigen Species
- Mouse
- Epitope
- Mouse IDO1 does not react with human tissues. Cross-reactivity with IDO1 from other sources has not been determined.
- Gene Name
- IDO1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 2E2.6
- Isotype
- IgG1
Immunogen
- Immunogen
- IDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Regulation of indoleamine 2, 3-dioxygenase in hippocampal microglia by NLRP3 inflammasome in lipopolysaccharide-induced depressive-like behaviors. Shanshan Zhang, Ying Zong, Zhonggan Ren, Juntao Hu, Xinyuan Wu, Honglei Xiao, Song Qin, Guomin Zhou, Yuanyuan Ma, Yaodong Zhang, Jin Yu, Kaidi Wang, Guocai Lu, Qiong Liu. The European journal of neuroscience. 2020 December;52:4586-4601. PubMed: 33098156
