Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
Western blot of Mouse Anti-IDO1 Antibody. Lane 1: untreated HeLa cells. Lane 2: IFN-r treated HeLa cells. Load: 35 ug per lane. Primary antibody: IDO 1 Antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 mouse secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 41-42 kDa, 41-42 kDa for IDO-1. Other band(s): none.
Monoclonal Mouse anti-Human IDO1 / IDO Antibody (clone 10.1, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C153780-100
Catalog Number: LS-C153780
For bulk orders or custom formulations:
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- Description
- IDO antibody LS-C153780 is an unconjugated mouse monoclonal antibody to IDO (IDO1) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC, IP and WB. Cited in 5 publications.
- Application
- ELISA, IF, IHC, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- IDO | Indole 2,3-dioxygenase | Indolamine 2,3 dioxygenase | IDO-1 | IDO1 | INDO | Indoleamine 2,3-dioxygenase | Indoleamine 2,3-dioxygenase 1
- Recommended Dilution: ELISA
- 1:5000 - 1:50000
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: IHC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:1500
- UniProt Number
- P14902
- NCBI GenBank
- AAA36081.1 | NM_002164
- SwissProt
- P14902
Target
- Target
- Human IDO1 / IDO
- Antigen Species
- Human
- Epitope
- This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined.
- Gene Name
- IDO1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 10.1
- Isotype
- IgG3
Immunogen
- Immunogen
- Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.09 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Synovial autoreactive T cells in rheumatoid arthritis resist IDO-mediated inhibition. Zhu L, Ji F, Wang Y, Zhang Y, Liu Q, Zhang JZ, Matsushima K, Cao Q, Zhang Y. Journal of immunology (Baltimore, Md. : 1950). 2006;177:8226-33.
PubMed: 17114500
Imaging correlates of differential expression of indoleamine 2,3-dioxygenase in human brain tumors. Batista CE, Juhsz C, Muzik O, Kupsky WJ, Barger G, Chugani HT, Mittal S, Sood S, Chakraborty PK, Chugani DC. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging. 11:460-6.
PubMed: 19434461 · PMC: PMC2763988
Psoriasis is characterized by deficient negative immune regulation compared to transient delayed-type hypersensitivity reactions. Gulati N, Suárez-Fariñas M, Correa da Rosa J, Krueger JG. F1000Research. 2015;4:149.
PubMed: 26236467 · PMC: PMC4505786
Indoleamine 2,3-dioxygenase regulates anti-tumor immunity in lung cancer by metabolic reprogramming of immune cells in the tumor microenvironment. Schafer CC, Wang Y, Hough KP, Sawant A, Grant SC, Thannickal VJ, Zmijewski J, Ponnazhagan S, Deshane JS. Oncotarget. 2016 November;7:75407-75424.
PubMed: 27705910 · PMC: PMC5340181
IL-32 induces indoleamine 2,3-dioxygenase+CD1c+ dendritic cells and indoleamine 2,3-dioxygenase+CD163+ macrophages: Relevance to mycosis fungoides progression. Ohmatsu H, Humme D, Gonzalez J, Gulati N, Möbs M, Sterry W, Krueger JG. Oncoimmunology. 2016 May;6:e1181237.
PubMed: 28344860 · PMC: PMC5353917
