Western Blot of Mouse Anti-IDO1 Antibody. Lane 1: untreated HeLa cells. Lane 2: IFN-r treated HeLa cells. Load: 35 µg per lane. Primary antibody: IDO 1 Antibody at 1:1000 for overnight at 4°C. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 41-42 kDa, 41-42 kDa for IDO-1. Other band(s): none.
IDO1 / IDO Antibody (clone 10.1)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744840-25
Catalog Number: LS-C744840
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- IDO antibody LS-C744840 is an unconjugated mouse monoclonal antibody to IDO (IDO1) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC, IP and WB.
- Application
- ELISA, IF, IHC, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- IDO | Indole 2,3-dioxygenase | Indolamine 2,3 dioxygenase | IDO-1 | IDO1 | INDO | Indoleamine 2,3-dioxygenase | Indoleamine 2,3-dioxygenase 1
- Recommended Dilution: ELISA
- 1:5000 - 1:50000
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: IHC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:1500
- UniProt Number
- P14902
- NCBI GenBank
- AAA36081.1 | NM_002164
- SwissProt
- P14902
Target
- Target
- Human IDO1 / IDO
- Antigen Species
- Human
- Epitope
- This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined.
- Gene Name
- IDO1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 10.1
- Isotype
- IgG3
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl