Western blot analysis of extracts of various cell lines, using HSPA5 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
HSPA5 / GRP78 / BiP Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C747004-20
Catalog Number: LS-C747004
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- BiP antibody LS-C747004 is an unconjugated rabbit polyclonal antibody to BiP (HSPA5 / GRP78) from human. It is reactive with human and mouse. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78 | HSPA5
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P11021
- NCBI GenBank
- AAA52614.1 | M19645
- SwissProt
- P11021
Target
- Target
- Human HSPA5 / GRP78 / BiP
- Antigen Species
- Human
- Gene Name
- HSPA5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant protein of human HSPA5
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS