HSPA5 Antibody western blot of HeLa,A2058,NCI-H460 cell line lysates (35 ug/lane). The HSPA5 antibody detected the HSPA5 protein (arrow).
HSPA5 / GRP78 / BiP Antibody (aa261-289)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Zebrafish |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C165064-400
Catalog Number: LS-C165064
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- Description
- BiP antibody LS-C165064 is an unconjugated rabbit polyclonal antibody to BiP (HSPA5 / GRP78) (aa261-289) from human. It is reactive with human and zebrafish. Validated for IF, IHC, Peptide-ELISA and WB. Cited in 1 publication.
- Application
- ELISA, IF, IHC, IHC-P, WB
- Reactivity
- Hu, Zf
- Predicted Reactivity
- Bovine, Chicken, Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78 | HSPA5
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P11021
- NCBI GenBank
- AAA52614.1 | M19645
- SwissProt
- P11021
Target
- Target
- Human HSPA5 / GRP78 / BiP
- Antigen Species
- Human
- Epitope
- This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-289 amino acids from the Central region of human HSPA5.
- Gene Name
- HSPA5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 0.5 mg/mL
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
Cell-extrinsic effects of tumor ER stress imprint myeloid dendritic cells and impair CD8 T cell priming. Mahadevan NR, Anufreichik V, Rodvold JJ, Chiu KT, Sepulveda H, Zanetti M. PloS one. 2012;7:e51845. PubMed: 23272178 PMC: PMC3525659