LSBio
IHC-plus™ Monoclonal Mouse anti-Human HSPA5 / GRP78 / BiP Antibody (clone 4E3, aa525-628, IHC, WB)

Anti-HSPA5 / GRP78 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

IHC-plus™ Monoclonal Mouse anti-Human HSPA5 / GRP78 / BiP Antibody (clone 4E3, aa525-628, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all HSPA5 / GRP78 / BiP antibodies →
$383.00each
Catalog Number: LS-B4157-50

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Overview

Description
BiP antibody LS-B4157 is an unconjugated mouse monoclonal antibody to human BiP (HSPA5 / GRP78) (aa525-628). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78 | HSPA5
Recommended Dilution: ELISA
1:10000
Recommended Dilution: IHC-P
1:200
Recommended Dilution: WB
1:100 - 1:500
UniProt Number
P11021
SwissProt
P11021

Target

Target
HSPA5 / GRP78 / BiP
Antigen Species
Human
Specificity
Human
Epitope
Human
Gene Name
HSPA5

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
4E3
Isotype
IgG1
Origin Species
Human

Immunogen

Immunogen
Purified recombinant fragment of human HSPA5 expressed in E. coli.
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Ascitic fluid, 0.03% sodium azide
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C.

Auxiliary proteins that facilitate formation of collagen-rich deposits in the posterior knee capsule in a rabbit-based joint contracture model. Andrzej Steplewski, Jolanta Fertala, Pedro K Beredjiklian , Joseph A Abboud , Mark L Y Wang , Surena Namdari , Jonathan Barlow , Michael Rivlin , William V Arnold , James Kostas, Cheryl Hou, Andrzej Fertala. The Breast : official journal of the European Society of Mastology. 2016 Mar;;34:489-501.

PubMed: 26241613


Endoplasmic reticulum-resident chaperones modulate the inflammatory and angiogenic responses of endothelial cells. R Tsaryk, N M Bartholomä, N Simiantonaki, L Anspach, K Peters, C Heilmann, C J Kirkpatrick, F Pröls. The British journal of dermatology. 2015 August;173:416-27.

PubMed: 25832777

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