The cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BIP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
HSPA5 / GRP78 / BiP Antibody (clone AT3D2)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C755485-50
Catalog Number: LS-C755485
For bulk orders or custom formulations:
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- Description
- BiP antibody LS-C755485 is an unconjugated mouse monoclonal antibody to human BiP (HSPA5 / GRP78). Validated for ELISA, Flow, ICC and WB.
- Application
- ELISA, FC, ICC, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78 | HSPA5
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P11021
- NCBI GenBank
- AAA52614.1, M19645
- SwissProt
- P11021
Target
- Target
- Human HSPA5 / GRP78 / BiP
- Antigen Species
- Hu
- Gene Name
- HSPA5
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- AT3D2
- Isotype
- IgG1
- Light Chain
- κ
- Origin Species
- Hu
Immunogen
- Immunogen
- Recombinant human BIP (20-650aa) purified from Baculo virus
- Immunogen Type
- Frag-RP
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Prot A
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS