WB of HSPA5 / GRP78 / BIP antibody. All lanes: Anti-HSPA5 at 0.5ug/ml. Lane 1: Rat Testis Tissue Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 72KD. Observed bind size: 72KD.
HSPA5 / GRP78 / BiP Antibody (aa603-617)
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Lyophilized |
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- Description
- BiP antibody LS-C312961 is an unconjugated rabbit polyclonal antibody to BiP (HSPA5 / GRP78) (aa603-617) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB. Cited in 2 publications.
- Application
- ICC, IHC, IHC-F, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78 | HSPA5
- UniProt Number
- P11021
- NCBI GenBank
- AAA52614.1 | M19645
- SwissProt
- P11021
Target
- Target
- Human HSPA5 / GRP78 / BiP
- Antigen Species
- Human
- Gene Name
- HSPA5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-Terminus of human GRP78 BiP(603-617 aa EWLESHQDADIEDFK), identical to the related mouse and rat sequences.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
- Concentration
- 0.5 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- NaCl
BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway. Ledet MM, Anderson R, Harman R, Muth A, Thompson PR, Coonrod SA, Van de Walle GR. BMC cancer. 2018 April;18:412.
PubMed: 29649984 · PMC: PMC5898062
Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation. Yeter Topçu-Tarladaçalisir, Melike Sapmaz-Metin, Zeynep Mercan, Deniz Erçetin. Balkan medical journal. 2024 January;41:30-37.
PubMed: 38173174 · PMC: PMC10767780