LSBio
Monoclonal Mouse anti-Rat HSPA5 / GRP78 / BiP Antibody (clone 10C3, aa649-654, IHC, WB)

Monoclonal Mouse anti-Rat HSPA5 / GRP78 / BiP Antibody (clone 10C3, aa649-654, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Fungus / Yeast / Mold, Rat
Format:Liquid
See all Rat HSPA5 / GRP78 / BiP antibodies →
$594.00each
Catalog Number: LS-C15345-50

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Overview

Description
BiP antibody LS-C15345 is an unconjugated mouse monoclonal antibody to BiP (HSPA5 / GRP78) (aa649-654) from rat. It is reactive with rat and yeast. Validated for ELISA, ICC, IHC, IP and WB.
Application
ELISA, ICC, IHC, IP, WB
Reactivity
Fung, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78 | HSPA5
Recommended Dilution: IHC
1:400
UniProt Number
P11021
SwissProt
P11021

Target

Target
Rat HSPA5 / GRP78 / BiP
Antigen Species
Rat
Epitope
Identifies purified Grp78 (BiP) protein and reacts with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 on SDS-PAGE immunoblots in samples from mammal, bird, insect and plant origin. Cross reacts with yeast proteins containing the HDEL sequence. An unidentified protein at ~55kD is also detected in some lysates. This antibody does not react with Hsp70 (Hsp72) or Hsc70 (Hsp73).
Gene Name
HSPA5

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
10C3
Isotype
IgG2a

Immunogen

Immunogen
A 6 residue synthetic peptide, SEKDEL, based on the rat Grp78 (aa 649-654), and the peptide coupled to KLH.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.