Detection of Human gamma-H2AX by Western Blot. Samples: Whole cell lysate (50 ug) from 293T cells that were mock treated (-) or treated with Etoposide (100 mu M, 2h). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
H2AFX / H2AX Antibody (phospho-Ser140)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287018-10
Catalog Number: LS-C287018
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- H2AX antibody LS-C287018 is an unconjugated rabbit polyclonal antibody to H2AX (H2AFX) (pSer140) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.
- Application
- ICC, IF, IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
- Recommended Dilution: IF/ICC
- 1:500 - 1:5000|1:1000 - 1:5000
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P16104
- NCBI GenBank
- NM_002105 | NP_002096.1
- SwissProt
- P16104
Target
- Target
- Human H2AFX / H2AX
- Antigen Species
- Human
- Epitope
- Region surrounding phosphorylated serine 140 of human histone H2AX using the numbering given in entry NP_002096.1 (GeneID 3014).
- Gene Name
- H2AX
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 3014 corresponding to amino acids surrounding serine 140 of UniprotID: P16104
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate