Western Blot of Rabbit anti-H2AXpS139 antibody. Lane 1: HeLa Lysate stimulated with Adriamycin (24 hr). Lane 2: HeLa Lysate unstimulated. Load: 35 ug per lane. Primary antibody: H2AXpS139 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 15.1 kDa, ~18 kDa for H2AXpS139. Other band(s): none.
H2AFX / H2AX Antibody (phospho-Ser139)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C209342-100
Catalog Number: LS-C209342
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- Description
- H2AX antibody LS-C209342 is an unconjugated rabbit polyclonal antibody to human H2AX (H2AFX) (pSer139). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
- Recommended Dilution: ELISA
- 1:5000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P16104
- NCBI GenBank
- NM_002105 | NP_002096.1
- SwissProt
- P16104
Target
- Target
- Human H2AFX / H2AX
- Antigen Species
- Human
- Gene Name
- H2AX
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-H2AX pS139 purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the C-terminal region containing serine 139 of human H2AX protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl