Anti-GPx4 Antibody - Western Blot. Western blot of affinity purified anti-GPx4 to detect GPx4 in testis extract (arrow). tissue extract (40 ug) was electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1000 dilution. Personal Communication, Dolph Hatfield, CCR-NCI, Bethesda, MD.
GPX4 / MCSP Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Guinea Pig, Mouse, Rat |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C18861-100
Catalog Number: LS-C18861
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- MCSP antibody LS-C18861 is an unconjugated rabbit polyclonal antibody to MCSP (GPX4) from mouse. It is reactive with mouse, rat and guinea pig. Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- GP, Ms, Rt
- Predicted Reactivity
- Human
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- GPX4 | GPx-4 | MCSP | SnPHGPx | Phospholipid hydroperoxidase | SnGPx | Glutathione peroxidase 4 | GSHPx-4 | PHGPx
- Recommended Dilution: ELISA
- 1:7000 - 1:30000
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- P36969
- NCBI GenBank
- NM_002085 | NP_002076.2
- SwissProt
- P36969
Target
- Target
- Mouse GPX4 / MCSP
- Antigen Species
- Mouse
- Epitope
- A BLAST analysis was used to suggest cross-reactivity with GPx4 protein from mouse, rat and guinea pig based on 100% homology with the immunizing sequence. Cross-reactivity with human GPx4 is also predicted based on a 94% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Gene Name
- GPX4
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.15 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl