Anti-Mouse NAG-1/GDF15 Antibody - Western Blot. Western blot of affinity purified anti-Mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kD) purified from E. coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kD) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kD) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4C. Nag-1 was detected using a 1:40000 dilution of peroxidase conjugated Gt-a-Rabbit antibody (LS-C60865) in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System. Other detection systems will yield similar results.