Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.

GDF15 Antibody (C-Terminus, HRP)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse
Format:	Lyophilized

$555.00each

Catalog Number: LS-C154280-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: GDF15 antibody LS-C154280 is an HRP-conjugated rabbit polyclonal antibody to GDF15 (C-Terminus) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Hu, Ms
Predicted Reactivity: Cynomolgus Monkey, Non-Human Primate
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: GDF15 | MIC1 | MIC-1 | NAG-1 | NRG-1 | NSAID-regulated gene 1 protein | PLAB | NSAID-activated gene 1 protein | Placental TGF-beta | PTGFB | GDF-15 | PTGF-beta
Recommended Dilution: ELISA: 1:10000 - 1:50000
Recommended Dilution: IHC: 1:500 - 1:2500
Recommended Dilution: WB: 1:1000 - 1:5000
UniProt Number: Q99988
NCBI GenBank: NM_004864 | NP_004855.2
SwissProt: Q99988

Target

Target: Human GDF15
Antigen Species: Human
Epitope: This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Gene Name: GDF15

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Anti-NAG-1 (C-terminal specific) antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Horseradish Peroxidase
Format: Lyophilized
Formulation: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration: 1.07 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl