Western Blot of Rabbit Anti-NAG-1 Biotin Conjugated Antibody. Lane 1: Rec. Human NAG1 (yeast expressed) reduced (0.1µg). Lane 2: Opal Prestained Molecular Weight Marker (5µL). Lane 3: Rec. Human NAG1 (yeast expressed) non-reduced (0.1µg). Primary Antibody: Anti-NAG1 Biotin Conjugate at 1µg/mL overnight at 2-8°C. Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30 min at RT.

GDF15 Antibody (Biotin)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse
Format:	Lyophilized

$564.00each

Catalog Number: LS-C154118-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: GDF15 antibody LS-C154118 is a biotin-conjugated rabbit polyclonal antibody to GDF15 from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: GDF15 | MIC1 | MIC-1 | NAG-1 | NRG-1 | NSAID-regulated gene 1 protein | PLAB | NSAID-activated gene 1 protein | Placental TGF-beta | PTGFB | GDF-15 | PTGF-beta
Recommended Dilution: ELISA: 1:20000 - 1:100000
Recommended Dilution: IHC: 1:1000 - 1:5000
Recommended Dilution: WB: 1:2000 - 1:10000
UniProt Number: Q99988
NCBI GenBank: NM_004864 | NP_004855.2
SwissProt: Q99988

Target

Target: Human GDF15
Antigen Species: Human
Epitope: This antibody reacts with endogenous NAG-1 protein from human tissues. A BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee based on 100% and 98% homology, respectively, and with macaque based on an 94% homology, and with mouse and rat based on 70% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Gene Name: GDF15

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to mature human NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography.
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label: Biotin
Format: Lyophilized
Formulation: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl