Positive WB detected in:Hela whole cell lysate,A549 whole cell lysate,HEK293 whole cell lysate,HepG2 whole cell lysate,Jurkat whole cell lysate,K562 whole cell lysate,Mouse liver tissue,Mouse kidney tissue;All lanes: GAPDH antibody at 2.5ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 37,32 kDa;Observed band size: 37,32 kDa;

GAPDH Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$300.00each

Catalog Number: LS-C498266-50

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Overview

Description: GAPDH antibody LS-C498266 is an unconjugated rabbit polyclonal antibody to GAPDH from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.
Application: ELISA, IF, IHC, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit | GAPDH
UniProt Number: P04406
NCBI GenBank: NM_002046 | NP_002037.2
SwissProt: P04406

Target

Target: Human GAPDH
Antigen Species: Human
Epitope: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Gene Name: GAPDH