Western blot analysis of Smac/Diablo expression in rat testis extract (lane 1), 22RV1 whole cell lysates (lane 2) and SKOV whole cell lysates (lane 3). Smac/Diablo at 25 kD was detected using rabbit anti- Smac/Diablo Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
DIABLO / SMAC Antibody (aa56-239)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C408072-100
Catalog Number: LS-C408072
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- Description
- SMAC antibody LS-C408072 is an unconjugated rabbit polyclonal antibody to SMAC (DIABLO) (aa56-239) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DFNA64 | DIABLO-S | DIABLO | Diablo homolog, mitochondrial | SMAC3 | Mitochondrial Smac protein | SMAC
- Recommended Dilution: ELISA
- 0.1 - 0.5 ug/ml
- UniProt Number
- Q9NR28
- NCBI GenBank
- NM_019887, NP_063940.1
- SwissProt
- Q9NR28
Target
- Target
- Human DIABLO / SMAC
- Antigen Species
- Hu
- Epitope
- Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. .
- Gene Name
- DIABLO
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
Immunogen
- Immunogen
- E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.
- Immunogen Type
- Recom
Format
- Conjugate/Label
- Unconj
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- NaCl