LSBio
Polyclonal Rabbit anti-Human DIABLO / SMAC Antibody (WB)

Western blot of anti-Smac detects a 26 kD band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 uM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. Antibody was used at a 1:1000 dilution.

Polyclonal Rabbit anti-Human DIABLO / SMAC Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human DIABLO / SMAC antibodies →
$579.00each
Catalog Number: LS-C19112-100

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Overview

Description
SMAC antibody LS-C19112 is an unconjugated rabbit polyclonal antibody to human SMAC (DIABLO). Validated for ELISA, IP and WB.
Application
ELISA, IHC, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DFNA64 | DIABLO-S | DIABLO | Diablo homolog, mitochondrial | SMAC3 | Mitochondrial Smac protein | SMAC
Recommended Dilution: ELISA
1:5000 - 1:20000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:1000 - 1:2000
UniProt Number
Q9NR28
NCBI GenBank
NM_019887 | NP_063940.1
SwissProt
Q9NR28

Target

Target
Human DIABLO / SMAC
Antigen Species
Human
Epitope
This antiserum is directed against human Smac/DIABLO and is useful in determining its presence in various assays.
Gene Name
DIABLO

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239).
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
85 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.