Western Blot: The cell lysate of Jurkat (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
DIABLO / SMAC Antibody (clone AT19F2)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C200849-50
Catalog Number: LS-C200849
For bulk orders or custom formulations:
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- Description
- SMAC antibody LS-C200849 is an unconjugated mouse monoclonal antibody to human SMAC (DIABLO). Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- DFNA64 | DIABLO-S | DIABLO | Diablo homolog, mitochondrial | SMAC3 | Mitochondrial Smac protein | SMAC
- Recommended Dilution: WB
- 1:3000
- UniProt Number
- Q9NR28
- NCBI GenBank
- NM_019887 | NP_063940.1
- SwissProt
- Q9NR28
Target
- Target
- Human DIABLO / SMAC
- Antigen Species
- Human
- Epitope
- Human DIABLO / SMAC
- Gene Name
- DIABLO
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- AT19F2
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Recombinant human SMAC/Diablo (56-239aa) purified from E. coli.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS