Western blot analysis of extracts of various cell lines, using CYBB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
CYBB / NOX2 / gp91phox Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C335167-20
Catalog Number: LS-C335167
For bulk orders or custom formulations:
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- Description
- Gp91phox antibody LS-C335167 is an unconjugated rabbit polyclonal antibody to gp91phox (CYBB / NOX2) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Chronic granulomatous disease | CGD91-phox | Cytochrome b558 subunit beta | Cytochrome b(558) subunit beta | CYBB | gp91-1 | gp91-PHOX | p22 phagocyte B-cytochrome | AMCBX2 | CGD | Cytochrome b-245 heavy chain | gp91PHOX | NADPH oxidase 2 | NOX2 | p91-PHOX
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P04839
- NCBI GenBank
- NM_000397 | NP_000388.2
- SwissProt
- P04839
Target
- Target
- Human CYBB / NOX2 / gp91phox
- Antigen Species
- Human
- Epitope
- Human NOX2 / gp91phox
- Gene Name
- CYBB
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CYBB (NP_000388.2). FHKMVAWMIALHSAIHTIAHLFNVEWCVNARVNNSDPYSVALSELGDRQNESYLNFARKRIKNPEGGLYLAVTLLAGITGVVITLCLILIITSSTKTIRRS
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS