MAN2A1 / Mannosidase II Antibody
Catalog Number: LS-C668190
4 sizes / from $272.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Anti-Collagen II Antibody - Immunohistochemistry. anti-collagen II antibody (1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Image provided courtesy of LifeSpan Biosciences, Seattle, WA.
Collagen II Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin |
| Reactivity: | Bovine, Human, Mammal (Other), Mouse, Rat, Sheep |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C18868-100
Catalog Number: LS-C18868
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Collagen II antibody LS-C18868 is an unconjugated rabbit polyclonal antibody to Collagen II from mammal. It is reactive with human, mouse, rat and other species. Validated for ELISA and IHC. Cited in 1 publication.
- Application
- ELISA, IHC, IHC-P
- Reactivity
- Bov, Hu, Mml, Ms, Rt, Sh
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:5000 - 1:50000
- Recommended Dilution: IHC-P
- 1:50 - 1:400
Target
- Target
- Mammal Collagen II
- Antigen Species
- Mammal (Other)
- Epitope
- Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Collagen Type II from human knee cartilage and bovine nasal cartilage.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
REAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model. Sanna Passino E, Rocca S, Caggiu S, Columbano N, Castagna A, Fontani V, Rinaldi S. Clinical interventions in aging. 2017 September;12:1471-1479. PubMed: 29066871 PMC: PMC5604553
