LSBio
Myosin II Antibody

Myosin II Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$568.00each
Catalog Number: LS-C84042-250

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Overview

Description
Myosin II antibody LS-C84042 is an unconjugated rabbit polyclonal antibody to Myosin II from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IP and WB. Cited in 1 publication.
Application
ICC, IF, IHC, IP, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: IF/ICC
1:500
Recommended Dilution: WB
1:5000

Target

Target
Human Myosin II
Antigen Species
Human
Epitope
This antibody recognizes myosin II and is highly specific for immunoblotting. Binding assays show strong reactivity with myosin heavy chain, weak reactivity with myosin light chain, and no cross-reactivity with skeletal and smooth muscle myosin. Immunocytochemistry shows uniform staining throughout growth cones in a punctate pattern with occasional concentration in filopodia. Rabbits were immunized with an intact and native myosin II purified from human platelets.

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Myosin II.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Phosphate buffered saline, pH 7.5, 0.015 M sodium phosphate, 0.15 M NaCl, 0.02% sodium azide
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
Phosphate buffered saline|0.015 M sodium phosphate|0.15 M NaCl
Functional role for stable microtubules in lens fiber cell elongation. Logan CM, Bowen CJ, Menko AS. Experimental cell research. 2018 January;362:477-488. PubMed: 29253534 PMC: PMC5764104
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