Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (20 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-Chk1 antibody used at the indicated concentrations. Detection: Chemiluminescence with exposure times of 15 seconds and 1 hour.
CHEK1 / CHK1 Antibody (aa250-300)
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| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287210-10
Catalog Number: LS-C287210
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- Description
- CHK1 antibody LS-C287210 is an unconjugated rabbit polyclonal antibody to human CHK1 (CHEK1) (aa250-300). Validated for IHC, IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CHEK1 | CHK1 checkpoint homolog | Cell cycle checkpoint kinase | Checkpoint kinase 1 | CHK1 | Chk1-S | Protein kinase chk1 | Checkpoint kinase-1
- Recommended Dilution: IHC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- O14757
- NCBI GenBank
- NM_001274 | NP_001265.2
- SwissProt
- O14757
Target
- Target
- Human CHEK1 / CHK1
- Antigen Species
- Human
- Epitope
- Region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in SwissProt entry O14757 (GeneID 1111).
- Gene Name
- CHEK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 1111 corresponding to amino acids between 250 and 300 of UniprotID: O14757
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate