Western blot analysis of extracts of various cell lines, using CDKN1A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
CDKN1A / WAF1 / p21 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C331492-20
Catalog Number: LS-C331492
For bulk orders or custom formulations:
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- Description
- P21 antibody LS-C331492 is an unconjugated rabbit polyclonal antibody to p21 (CDKN1A / WAF1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CDKN1 | CIP1 | CDK-interacting protein 1 | CDKN1A | DNA synthesis inhibitor | p21CIP1 | MDA6 | PIC1 | SDI1 | WAF1 | CAP20 | CDK-interaction protein 1 | MDA-6 | p21
- Recommended Dilution: IF/ICC
- 1:20 - 1:100
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P38936
- NCBI GenBank
- NM_078467 | NP_000380.1
- SwissProt
- P38936
Target
- Target
- Human CDKN1A / WAF1 / p21
- Antigen Species
- Human
- Epitope
- Human CDKN1A / WAF1 / p21
- Gene Name
- CDKN1A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant protein of human CDKN1A
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS with 0.09% Sodium azide,50% glycerol,pH7.3
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS