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Monoclonal Mouse anti-Human CDKN1A / WAF1 / p21 Antibody (clone WA-1, Concentrated, IHC, WB)

Monoclonal Mouse anti-Human CDKN1A / WAF1 / p21 Antibody (clone WA-1, Concentrated, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human CDKN1A / WAF1 / p21 antibodies →
$441.00each
Catalog Number: LS-C202479-100

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Overview

Description
P21 antibody LS-C202479 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for ELISA, Flow, IHC and WB.
Application
ELISA, FC, IHC, IHC-F, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CDKN1 | CIP1 | CDK-interacting protein 1 | CDKN1A | DNA synthesis inhibitor | p21CIP1 | MDA6 | PIC1 | SDI1 | WAF1 | CAP20 | CDK-interaction protein 1 | MDA-6 | p21
UniProt Number
P38936
SwissProt
P38936

Target

Target
Human CDKN1A / WAF1 / p21
Antigen Species
Human
Epitope
Clone WA-1 reacts with human and other mammalian p21 protein. The intracellular protein p21 is a 21 kD protein and also known as wild-type p53-activated fragment 1 (WAF1). It is an inhibitor of cyclin-dependent kinases (Cdks) and proliferating-cell nuclear antigen (PCNA). Source: A BALB/c mouse was immunized with human p21 protein. Splenocytes were fused with mouse myeloma cell line SP2. Hybridomas were selected in DMEM supplied with 20% FCS, 1gg/ml azaserine and 10-4 M hypoxanthine. The immunoglobulin producing hybridoma with desired specificity was cloned twice by limiting dilution.
Gene Name
CDKN1A

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
WA-1
Isotype
IgG1

Immunogen

Immunogen
Human p21 protein. Splenocytes were fused with mouse myeloma cell line SP2. Hybridomas were selected in DMEM supplied with 20% FCS, 1 ug/ml azaserine and 10-4 M hypoxanthine. The immunoglobulin producing hybridoma with desired specificity was cloned twice by limiting dilution.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.05% Sodium Azide
Concentration
100 µg/mL
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store at 2-8°C.
Recommended Storage Buffer
PBS

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