LSBio
IHC-plus™ CDKN1A / WAF1 / p21 Antibody (aa111-160)

Anti-CDKN1A / WAF1 / p21 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ CDKN1A / WAF1 / p21 Antibody (aa111-160)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$449.00each
Catalog Number: LS-B9242-50

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Overview

Description
P21 antibody LS-B9242 is an unconjugated rabbit polyclonal antibody to p21 (CDKN1A / WAF1) (aa111-160) from human. It is reactive with human, mouse and rat. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CDKN1 | CIP1 | CDK-interacting protein 1 | CDKN1A | DNA synthesis inhibitor | p21CIP1 | MDA6 | PIC1 | SDI1 | WAF1 | CAP20 | CDK-interaction protein 1 | MDA-6 | p21
Recommended Dilution: IHC-P
1:50
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P38936
SwissProt
P38936

Target

Target
CDKN1A / WAF1 / p21
Antigen Species
Human
Specificity
aa111-160 of human, mouse, rat CDKN1A protein
Epitope
CDKN1A Antibody detects endogenous levels of total CDKN1A protein.
Gene Name
CDKN1A

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from human p21 Cip1. (AA Range: 111-160)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year.
Recommended Storage Buffer
PBS, 150 mM NaCl
Expression levels of hnRNP K and p21WAF1/CIP1 are associated with resistance to radiochemotherapy independent of p53 pathway activation in rectal adenocarcinoma. Daskalaki W, Wardelmann E, Port M, Stock K, Steinestel J, Huss S, Sperveslage J, Steinestel K, Eder S. International journal of molecular medicine. 2018 December;42:3269-3277. PubMed: 30272263 PMC: PMC6202084

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