Anti-Human p21WAF1 Monoclonal Antibody - Western Blot. Mab anti-Human p21WAF1 antibody (clone WA-1) is shown to detect human p21 by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human p21WAF1at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] ( diluted 1:5000. A single band corresponding to human p21WAF1 is detected at ~21 kD when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p21WAF1. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
CDKN1A / WAF1 / p21 Antibody (clone WA-1)
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
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- Description
- P21 antibody LS-C59514 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for ELISA, IF, IHC, IP and WB. Cited in 5 publications.
- Application
- ELISA, IF, IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CDKN1 | CIP1 | CDK-interacting protein 1 | CDKN1A | DNA synthesis inhibitor | p21CIP1 | MDA6 | PIC1 | SDI1 | WAF1 | CAP20 | CDK-interaction protein 1 | MDA-6 | p21
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IF/ICC
- 1:250
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P38936
- NCBI GenBank
- NM_078467 | NP_000380.1
- SwissProt
- P38936
Target
- Target
- Human CDKN1A / WAF1 / p21
- Antigen Species
- Human
- Gene Name
- CDKN1A
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- WA-1
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.5 M NaCl
The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo. Yang ZY, Perkins ND, Ohno T, Nabel EG, Nabel GJ. Nature medicine. 1995;1:1052-6.
PubMed: 7489362
p21 is necessary for the p53-mediated G1 arrest in human cancer cells. Waldman T, Kinzler KW, Vogelstein B. Cancer research. 1995;55:5187-90.
PubMed: 7585571
A cis-acting element in Rous sarcoma virus long terminal repeat required for promoter repression by HeLa nuclear protein p21. Yeh CH, Shatkin AJ. The Journal of biological chemistry. 1995;270:15815-20.
PubMed: 7797584
p53 expression overcomes p21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells. Kagawa S, Fujiwara T, Hizuta A, Yasuda T, Zhang WW, Roth JA, Tanaka N. Oncogene. 1997;15:1903-9.
PubMed: 9365236
Inhibition of tumor cell growth by p21WAF1 adenoviral gene transfer in lung cancer. Joshi US, Chen YQ, Kalemkerian GP, Adil MR, Kraut M, Sarkar FH. Cancer gene therapy. 5:183-91.
PubMed: 9622102