Flow cytometry of rat splenocytes with Mouse anti-Rat CD86:RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.

CD86 Antibody (clone 24F, RPE)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Flow Cytometry
Reactivity:	Rat
Format:	Lyophilized

$438.00each

Catalog Number: LS-C188425-100

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Overview

Description: CD86 antibody LS-C188425 is an RPE-conjugated mouse monoclonal antibody to rat CD86. Validated for Flow.
Application: FC
Reactivity: Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: Activation B7-2 antigen | B7-2 | B7.2 | B70 | CD86 molecule | CD86 | CTLA-4 counter-receptor B7.2 | CD86 antigen | FUN-1 | LAB72 | BU63 | CD28LG2 | Ctla-4 counter-receptors b7.2
Recommended Dilution: FC: 0.0423611111111111
UniProt Number: P42081
NCBI GenBank: NM_006889 | NP_008820.3
SwissProt: P42081

Target

Target: Rat CD86
Antigen Species: Rat
Epitope: Recognizes rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages. Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production. Clone 24F has been shown to block the co-stimulatory activity of rat CD86.
Gene Name: CD86

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 24F
Isotype: IgG1

Immunogen

Immunogen: HTLV-1 transformed Lewis - S1 cells.
Immunogen Type: Cell-Based Immunization

Format

Conjugate/Label: Phycoerythrin
Format: Lyophilized
Formulation: PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Preservative: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer: PBS