Flow cytometric analysis of rat CD86 expression on LPS-stimulated SD rat splenocytes. Cells were stained with purified anti-rat CD86, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

CD86 Antibody (clone 10, Preservative Free)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry
Reactivity:	Rat
Format:	Liquid

$148.00each

Catalog Number: LS-C820335-20

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: CD86 antibody LS-C820335 is an unconjugated mouse monoclonal antibody to rat CD86. Validated for ELISA and Flow.
Application: ELISA, FC
Reactivity: Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: Activation B7-2 antigen | B7-2 | B7.2 | B70 | CD86 molecule | CD86 | CTLA-4 counter-receptor B7.2 | CD86 antigen | FUN-1 | LAB72 | BU63 | CD28LG2 | Ctla-4 counter-receptors b7.2
Recommended Dilution: ELISA: 1:1000 - 1:10000
Recommended Dilution: FC: 1:25 - 1:100
UniProt Number: P42081
NCBI GenBank: NM_006889 | NP_008820.3
SwissProt: P42081

Target

Target: Rat CD86
Antigen Species: Rat
Epitope: Rat CD86
Gene Name: CD86

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 10
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant Rat CD86 / B7-2 protein
Immunogen Type: Recombinant Immunogen

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.2 µm filtered solution in PBS
Volume: 20 Microliter
Preservative: No
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS