Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% Triton X-100 (3 min, room temperature). Cells were incubated with a final concentration of 10 mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD144 / CDH5 / VE Cadherin Antibody (clone BV9)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$566.00each
Catalog Number: LS-C139964-100
Catalog Number: LS-C139964
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- Description
- VE Cadherin antibody LS-C139964 is an unconjugated mouse monoclonal antibody to human VE Cadherin (CD144 / CDH5). Validated for Flow, Func, IF, IHC, IP and WB.
- Application
- FC, Func, IF, IHC, IHC-F, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 7B4 | 7B4 antigen | CD144 | CDH5 | Cadherin-5 | Endothelial-specific cadherin | Vascular endothelial cadherin | VE-cadherin | VEC | CD144 antigen | VE Cadherin
- UniProt Number
- P33151
- NCBI GenBank
- NM_001795 | NP_001786.2
- SwissProt
- P33151
Target
- Target
- Human CD144 / CDH5 / VE Cadherin
- Antigen Species
- Human
- Epitope
- Human CDH5 / VE Cadherin
- Gene Name
- CDH5
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- BV9
- Isotype
- IgG2a
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.1% BSA
- Concentration
- 0.1 mg/mL
- Preservative
- No
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store at 4°C, stable for 1 year.
- Recommended Storage Buffer
- PBS