HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAST (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAST.
CAST / Calpastatin Antibody (clone OTI1E7)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C337646-100
Catalog Number: LS-C337646
For bulk orders or custom formulations:
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- Description
- Calpastatin antibody LS-C337646 is an unconjugated mouse monoclonal antibody to human Calpastatin (CAST). Validated for Flow, IHC and WB.
- Application
- FC, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Calpastatin | BS-17 | Calpain inhibitor | Sperm BS-17 component | CAST
- Recommended Dilution: FC
- 1:100
- Recommended Dilution: IHC-P
- 1:150
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- P20810
- NCBI GenBank
- NM_001750 | NP_001741.4
- SwissProt
- P20810
Target
- Target
- Human CAST / Calpastatin
- Antigen Species
- Human
- Epitope
- Human CAST / Calpastatin
- Gene Name
- CAST
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI1E7
- Isotype
- IgG1
Immunogen
- Immunogen
- Full length human recombinant protein of human CAST(NP_001741) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 0.62 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS