LSBio
Alpha-Glucosidase Assay Kit (Colorimetric)

Alpha-Glucosidase Assay Kit (Colorimetric)

Assay FormatEnzyme Activity Assay
Format:Kit
See all Alpha-Glucosidase antibodies →
$597.00each
Catalog Number: LS-K194-100

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Overview

Description
Alpha-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked Alpha-D-glucose residues with release of Alpha-D-glucose. Alpha-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring Alpha-glucosidase activity are becoming popular in Research and Drug Discovery. This Alpha-Glucosidase Assay Kit is designed to measure Alpha-glucosidase activity directly in biological samples without pretreatment. The improved Method utilizes p-nitrophenyl-Alpha-D-glucopyranoside that is hydrolyzed specifically by Alpha-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
Target
Alpha-Glucosidase
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Enzyme Activity Assay
Compatible Sample Types
Other Biological Sample
Detection Wavelength (Colorimetric)
405 nm

Format

Format
Kit
Kit Components
Assay Buffer (pH 7.0), a-NPG Substrate, Calibrator (equivalent to 250 U\/L)

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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