
Alpha-Glucosidase Assay Kit (Colorimetric)
| Assay Format | Enzyme Activity Assay |
| Format: | Kit |
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- Description
- Alpha-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked Alpha-D-glucose residues with release of Alpha-D-glucose. Alpha-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring Alpha-glucosidase activity are becoming popular in Research and Drug Discovery. This Alpha-Glucosidase Assay Kit is designed to measure Alpha-glucosidase activity directly in biological samples without pretreatment. The improved Method utilizes p-nitrophenyl-Alpha-D-glucopyranoside that is hydrolyzed specifically by Alpha-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
- Target
- Alpha-Glucosidase
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Colorimetric
- Assay Type
- Quantitative
- Assay Format
- Enzyme Activity Assay
- Compatible Sample Types
- Other Biological Sample
- Detection Wavelength (Colorimetric)
- 405 nm
Format
- Format
- Kit
- Kit Components
- Assay Buffer (pH 7.0), a-NPG Substrate, Calibrator (equivalent to 250 U\/L)
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document




















