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Polyclonal Rabbit anti-Human HAUS8 Antibody (WB)

Anti-HICE1 in Western blot of Immunochemicals' Anti-HICE1 Antibody shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HICE1. In right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.

Polyclonal Rabbit anti-Human HAUS8 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human HAUS8 antibodies →
$579.00each
Catalog Number: LS-C153765-100

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Overview

Description
HAUS8 antibody LS-C153765 is an unconjugated rabbit polyclonal antibody to human HAUS8. Validated for ELISA and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Predicted Reactivity
NHP
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DGT4 | HAUS8 | NY-SAR-48 | Sarcoma antigen NY-SAR-48 | HICE1
Recommended Dilution: ELISA
1:250000
Recommended Dilution: WB
1:10000
UniProt Number
Q9BT25
SwissProt
Q9BT25

Target

Target
Human HAUS8
Antigen Species
Hu
Epitope
This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology with the immunizing sequence. Expect reactivity with Hice1 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence. Cross-reactivity with Hice1 from other sources has not been determined.
Gene Name
HAUS8

Antibody

Host Species
Rb
Clonality
Polyclonal

Immunogen

Immunogen
Anti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.
Immunogen Type
FL-RP

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
38 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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