LSBio
GAPDH Antibody (clone 1653CT401.3.33)

All lanes: Anti-GAPDH Antibody at 1:8000 dilution Lane 1: C6 whole cell lysate Lane 2: mouse brain lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

GAPDH Antibody (clone 1653CT401.3.33)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human GAPDH antibodies →
$401.00each
Catalog Number: LS-C757900-200

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Overview

Description
GAPDH antibody LS-C757900 is an unconjugated mouse monoclonal antibody to GAPDH from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
Application
ELISA, FC, IF, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit | GAPDH
Recommended Dilution: FC
1:25
Recommended Dilution: IF/ICC
1:25
Recommended Dilution: IHC-P
1:25
Recommended Dilution: WB
1:8000
UniProt Number
P04406
SwissProt
P04406

Target

Target
Human GAPDH
Antigen Species
Human
Gene Name
GAPDH

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
1653CT401.3.33
Isotype
IgG1
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
This GAPDH antibody is generated from a mouse immunized with a recombinant protein between 43-335 amino acids from human GAPDH.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Volume
200 Microliter
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.