Human COPA / Xenin

Rabbit

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide: M(1)LTKFETKSARVKGLSFHP(19)C. Immunizing peptide corresponds to amino acid residues 1-19 from rat COP I alpha. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Dog, Horse, Pig, Opossum, Chicken, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish, Seq squirt, Ant, Water flea, Tick (100%); Mosquito, Sea anemone, Rice, Beetle, Arabidopsis, Spikemoss (94%); Drosophila, Trichoplax, Grape, Castor bean (89%).

aa1-19

Detects rat coatomer-protein I alpha (COP I alpha). Species cross-reactivity: Human.

• IHC
• IHC - Paraffin (5 µg/ml)
• ICC
• Western blot (1 µg/ml)

Immunohistochemistry: LS-B2566 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2566 was determined to be 5 ug/ml. Western Blot: 1 ug/ml, detects an ~140kD protein representing COPI alpha from PC-12 cell extract. Also detects an unidentified protein at ~100kD.

PBS, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P53621 NCBI: NM_004371 NP_004362.2