Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COPA / Xenin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119195-100
Catalog Number: LS-G119195
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COPA (NM_001098398), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha coat protein | Alpha-COP | Alpha COP I | Alpha-coat protein | Coatomer alpha subunit | Coatomer subunit alpha | COPA | HEPCOP | HEP-COP | Xenin
- Origin Species
- Human
- NCBI GenBank
- NM_004371
- SwissProt
- P53621
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 139.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLTKFETKSA RVKGLSFHPK RPWILTSLHN GVIQLWDYRM CTLIDKFDEH DGPVRGIDFH61KQQPLFVSGG DDYKIKVWNY KLRRCLFTLL GHLDYIRTTF FHHEYPWILS ASDDQTIRVW121NWQSRTCVCV LTGHNHYVMC AQFHPTEDLV VSASLDQTVR VWDISGLRKK NLSPGAVESD181VRGITGVDLF GTTDAVVKHV LEGHDRGVNW AAFHPTMPLI VSGADDRQVK IWRMNESKAW241EVDTCRGHYN NVSCAVFHPR QELILSNSED KSIRVWDMSK RTGVQTFRRD HDRFWVLAAH301PNLNLFAAGH DGGMIVFKLE RERPAYAVHG NMLHYVKDRF LRQLDFNSSK DVAVMQLRSG361SKFPVFNMSY NPAENAVLLC TRASNLENST YDLYTIPKDA DSQNPDAPEG KRSSGLTAVW421VARNRFAVLD RMHSLLIKNL KNEITKKVQV PNCDEIFYAG TGNLLLRDAD SITLFDVQQK481RTLASVKISK VKYVIWSADM SHVALLAKHE HSCPLPLTAI VICNRKLDAL CNIHENIRVK541SGAWDESGVF IYTTSNHIKY AVTTGDHGII RTLDLPIYVT RVKGNNVYCL DRECRPRVLT601IDPTEFKFKL ALINRKYDEV LHMVRNAKLV GQSIIAYLQK KGYPEVALHF VKDEKTRFSL661ALECGNIEIA LEAAKALDDK NCWEKLGEVA LLQGNHQIVE MCYQRTKNFD KLSFLYLITG721NLEKLRKMMK IAEIRKDMSG HYQNALYLGD VSERVRILKN CGQKSLAYLT AATHGLDEEA781ESLKETFDPE KETIPDIDPN AKLLQPPAPI MPLDTNWPLL TVSKGFFEGT IASKGKGGAL841AADIDIDTVG TEGWGEDAEL QLDEDGFVEA TEGLGDDALG KGQEEGGGWD VEEDLELPPE901LDISPGAAGG AEDGFFVPPT KGTSPTQIWC NNSQLPVDHI LAGSFETAMR LLHDQVGVIQ961FGPYKQLFLQ TYARGRTTYQ ALPCLPSMYG YPNRNWKDAG LKNGVPAVGL KLNDLIQRLQ1021LCYQLTTVGK FEEAVEKFRS ILLSVPLLVV DNKQEIAEAQ QLITICREYI VGLSVETERK1081KLPKETLEQQ KRICEMAAYF THSNLQPVHM ILVLRTALNL FFKLKNFKTA ATFARRLLEL1141GPKPEVAQQT RKILSACEKN PTDAYQLNYD MHNPFDICAA SYRPIYRGKP VEKCPLSGAC1201YSPEFKGQIC RVTTVTEIGK DVIGLRISPL QFRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1233 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.