LSBio
Human COPA / Xenin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human COPA / Xenin Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all COPA antibodies →
$220.00each
Catalog Number: LS-G87502-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human COPA (NM_001098398) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Alpha coat protein | Alpha-COP | Alpha COP I | Alpha-coat protein | Coatomer alpha subunit | Coatomer subunit alpha | COPA | HEPCOP | HEP-COP | Xenin
Origin Species
Human
NCBI GenBank
NM_004371
SwissProt
P53621

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
139.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLTKFETKSA RVKGLSFHPK RPWILTSLHN GVIQLWDYRM CTLIDKFDEH DGPVRGIDFH
61KQQPLFVSGG DDYKIKVWNY KLRRCLFTLL GHLDYIRTTF FHHEYPWILS ASDDQTIRVW
121NWQSRTCVCV LTGHNHYVMC AQFHPTEDLV VSASLDQTVR VWDISGLRKK NLSPGAVESD
181VRGITGVDLF GTTDAVVKHV LEGHDRGVNW AAFHPTMPLI VSGADDRQVK IWRMNESKAW
241EVDTCRGHYN NVSCAVFHPR QELILSNSED KSIRVWDMSK RTGVQTFRRD HDRFWVLAAH
301PNLNLFAAGH DGGMIVFKLE RERPAYAVHG NMLHYVKDRF LRQLDFNSSK DVAVMQLRSG
361SKFPVFNMSY NPAENAVLLC TRASNLENST YDLYTIPKDA DSQNPDAPEG KRSSGLTAVW
421VARNRFAVLD RMHSLLIKNL KNEITKKVQV PNCDEIFYAG TGNLLLRDAD SITLFDVQQK
481RTLASVKISK VKYVIWSADM SHVALLAKHE HSCPLPLTAI VICNRKLDAL CNIHENIRVK
541SGAWDESGVF IYTTSNHIKY AVTTGDHGII RTLDLPIYVT RVKGNNVYCL DRECRPRVLT
601IDPTEFKFKL ALINRKYDEV LHMVRNAKLV GQSIIAYLQK KGYPEVALHF VKDEKTRFSL
661ALECGNIEIA LEAAKALDDK NCWEKLGEVA LLQGNHQIVE MCYQRTKNFD KLSFLYLITG
721NLEKLRKMMK IAEIRKDMSG HYQNALYLGD VSERVRILKN CGQKSLAYLT AATHGLDEEA
781ESLKETFDPE KETIPDIDPN AKLLQPPAPI MPLDTNWPLL TVSKGFFEGT IASKGKGGAL
841AADIDIDTVG TEGWGEDAEL QLDEDGFVEA TEGLGDDALG KGQEEGGGWD VEEDLELPPE
901LDISPGAAGG AEDGFFVPPT KGTSPTQIWC NNSQLPVDHI LAGSFETAMR LLHDQVGVIQ
961FGPYKQLFLQ TYARGRTTYQ ALPCLPSMYG YPNRNWKDAG LKNGVPAVGL KLNDLIQRLQ
1021LCYQLTTVGK FEEAVEKFRS ILLSVPLLVV DNKQEIAEAQ QLITICREYI VGLSVETERK
1081KLPKETLEQQ KRICEMAAYF THSNLQPVHM ILVLRTALNL FFKLKNFKTA ATFARRLLEL
1141GPKPEVAQQT RKILSACEKN PTDAYQLNYD MHNPFDICAA SYRPIYRGKP VEKCPLSGAC
1201YSPEFKGQIC RVTTVTEIGK DVIGLRISPL QFR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1233 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.