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Anti-CASZ1 Antibody (N-Terminus) IHC-plus™ LS-B2838

Note: This antibody replaces LS-C60095
Catalog Size Price
LS-B2838-50 50 µg (1 mg/ml) Unavailable

Related Products

13 CASZ1 Antibodies

Most Popular CASZ1 Antibodies

Anti-CASZ1 Antibody (N-Terminus) IHC-plus™ LS-B2838
Rabbit Polyclonal to Human CASZ1
Human, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CASZ1 Antibody (Internal) LS-C154253
Rabbit Polyclonal (IgG) to Human CASZ1
Human, Mouse, Drosophila, Macaque
Western blot, ELISA
Unconjugated
Western blot Image
Anti-CASZ1 Antibody (aa1574-1601, AP) LS-C233388
Rabbit Polyclonal (IgG) to Human CASZ1
Human, Mouse
Western blot, ELISA
AP Conjugated
Anti-CASZ1 Antibody (aa1574-1601, APC) LS-C233389
Rabbit Polyclonal (IgG) to Human CASZ1
Human, Mouse
Western blot, ELISA
APC Conjugated
Anti-CASZ1 Antibody (aa1581-1609) LS-C320012
Rabbit Polyclonal (IgG) to Human CASZ1
Human, Mouse
Western blot, ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal to Human CASZ1
Human, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CASZ1
Rabbit
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:3000)
  • ELISA (1:25000 - 1:100000)

Specificity and Use

CASZ1 antibody was raised against synthetic peptide located between amino acids 1-150 of B3KRV8
N-Terminus
human Cas5 protein. A BLAST analysis was used to suggest cross reactivity with Cas5 from mouse, chimpanzee, bovine, and rat based on 100% homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross reactivity with Cas5 from other sources has not been determined
Immunohistochemistry: LS-B2838 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2838 was determined to be 5 ug/ml. Western Blot: A band approximately 125 kD in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About CASZ1

Q86V15 NM_017766 NP_060236.3

CASZ1 Antibody, Castor-related protein Antibody, CAS11 Antibody, Castor Antibody, Castor homolog 1, zinc finger Antibody, CST Antibody, DJ734G22.1 Antibody, Zinc finger protein 693 Antibody, SRG Antibody, ZNF693 Antibody, Castor zinc finger 1 Antibody, Survival-related Antibody

Transcription factor involved in vascular assembly and morphogenesis through direct transcriptional regulation of EGFL7.

Immunohistochemistry

Immunohistochemistry
Anti-CASZ1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2838 concentration 5 ug/ml.

Western blot

Western blot
Anti-hCas5 Antibody - Western Blot. Western blot of affinity purified anti-hCas5 antibody shows detection of a band ~125 kD in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kD). Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA. Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication. Carol Thiele, NCI, Bethesda, MD.

Requested From: 
Date Requested: 8/22/2017

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