Anti-hCASZ1 Antibody - Western Blot. Western blot of anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 ug). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kD). Lane 4, SY5Y transfected with hCASZ11 (190kD). Personal communication, Carol Thiele, NCI, Bethesda, MD.

CASZ1 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Cynomolgus Monkey
Format:	Liquid

$579.00each

Catalog Number: LS-C154253-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: CASZ1 antibody LS-C154253 is an unconjugated rabbit polyclonal antibody to CASZ1 from human. It is reactive with human, mouse, chimpanzee and other species. Validated for ELISA, IF, IHC and WB.
Application: ELISA, IF, IHC, WB
Reactivity: Cyno
Predicted Reactivity: Cynomolgus Monkey, Fruit Fly, Human, Mouse, Non-Human Primate
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Castor-related protein | CAS11 | Castor | Castor homolog 1, zinc finger | CST | CASZ1 | DJ734G22.1 | Zinc finger protein 693 | SRG | ZNF693 | Castor zinc finger 1 | Survival-related
Recommended Dilution: ELISA: 1:45000
Recommended Dilution: WB: 1:1000 - 1:5000
UniProt Number: Q86V15
NCBI GenBank: NM_017766 | NP_060236.3
SwissProt: Q86V15

Target

Target: Human CASZ1
Antigen Species: Human
Epitope: A BLAST analysis was used to suggest reactivity with CASZ1 proteins from human, mouse, Drosophila, chimpanzee, and macaque based on a 100% homology. Partial reactivity is expected with horse and dog CASZ1 based on a 92% homology with the immunizing sequence. Cross-reactivity with CASZ1 from other sources has not been determined.
Gene Name: CASZ1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1.07 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl