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CASZ1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CASZ1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112240-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CASZ1 (NM_017766), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Castor-related protein | CAS11 | Castor | Castor homolog 1 | zinc finger | CST | CASZ1 | DJ734G22.1 | Zinc finger protein 693 | SRG | ZNF693 | Castor zinc finger 1 | Survival-related
Origin Species
Human
NCBI GenBank
NM_017766
SwissProt
Q86V15

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLGTAEGTR CTDPPAGKPA MAPKRKGGLK LNAICAKLSR QVVVEKRADA GSHTEGSPSQ
61PRDQERSGPE SGAARAPRSE EDKRRAVIEK WVNGEYSEEP APTPVLGRIA REGLELPPEG
121VYMVQPQGCS DEEDHAEEPS KDGGALEEKD SDGAASKEDS GPSTRQASGE ASSLRDYAAS
181TMTEFLGMFG YDDQNTRDEL ARKISFEKLH AGSTPEAATS SMLPTSEDTL SKRARFSKYE
241EYIRKLKAGE QLSWPAPSTK TEERVGKEVV GTLPGLRLPS STAHLETKAT ILPLPSHSSV
301QMQNLVARAS KYDFFIQKLK TGENLRPQNG STYKKPSKYD LENVKYLHLF KPGEGSPDMG
361GAIAFKTGKV GRPSKYDVRG IQKPGPAKVP PTPSLAPAPL ASVPSAPSAP GPGPEPPASL
421SFNTPEYLKS TFSKTDSITT GTVSTVKNGL PTDKPAVTED VNIYQKYIAR FSGSQHCGHI
481HCAYQYREHY HCLDPECNYQ RFTSKQDVIR HYNMHKKRDN SLQHGFMRFS PLDDCSVYYH
541GCHLNGKSTH YHCMQVGCNK VYTSTSDVMT HENFHKKNTQ LINDGFQRFR ATEDCGTADC
601QFYGQKTTHF HCRRPGCTFT FKNKCDIEKH KSYHIKDDAY AKDGFKKFYK YEECKYEGCV
661YSKATNHFHC IRAGCGFTFT STSQMTSHKR KHERRHIRSS GALGLPPSLL GAKDTEHEES
721SNDDLVDFSA LSSKNSSLSA SPTSQQSSAS LAAATAATEA GPSATKPPNS KISGLLPQGL
781PGSIPLALAL SNSGLPTPTP YFPILAGRGS TSPPVGTPSL LGAVSSGSAA SATPDTPTLV
841ASGAGDSAPV AAASVPAPPA SIMERISASK GLISPMMARL AAAALKPSAT FDPGSGQQVT
901PARFPPAQVK PEPGESTGAP GPHEASQDRS LDLTVKEPSN ESNGHAVPAN SSLLSSLMNK
961MSQGNPGLGS LLNIKAEAEG SPAAEPSPFL GKAVKALVQE KLAEPWKVYL RRFGTKDFCD
1021GQCDFLHKAH FHCVVEECGA LFSTLDGAIK HANFHFRTEG GAAKGNTEAA FPASAAETKP
1081PMAPSSPPVP PVTTATVSSL EGPAPSPASV PSTPTLLAWK QLASTIPQMP QIPASVPHLP
1141ASPLATTSLE NAKPQVKPGF LQFQEK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1166 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.