Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEMA4A / Semaphorin 4A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G131062-100
Catalog Number: LS-G131062
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEMA4A (NM_001193302), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CORD10 | RP11-54H19.2 | RP35 | Sema B | Semaphorin-4A | Semaphorin-B | SEMB | SEMA4A | SEMAB
- Origin Species
- Human
- NCBI GenBank
- NM_022367
- SwissProt
- Q9H3S1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIPWPASDRK KSECAFKKKS NEELQDSYLL PISEDKVMEG KGQSPFDPAH KHTAVLVDGM61LYSGTMNNFL GSEPILMRTL GSQPVLKTDN FLRWLHHDAS FVAAIPSTQV VYFFFEETAS121EFDFFERLHT SRVARVCKND VGGEKLLQKK WTTFLKAQLL CTQPGQLPFN VIRHAVLLPA181DSPTAPHIYA VFTSQWQVGG TRSSAVCAFS LLDIERVFKG KYKELNKETS RWTTYRGPET241NPRPGSCSVG PSSDKALTFM KDHFLMDEQV VGTPLLVKSG VEYTRLAVET AQGLDGHSHL301VMYLGTTTGS LHKAVVSGDS SAHLVEEIQL FPDPEPVRNL QLAPTQGAVF VGFSGGVWRV361PRANCSVYES CVDCVLARDP HCAWDPESRT CCLLSAPNLN SWKQDMERGN PEWACASGPM421SRSLRPQSRP QIIKEVLAVP NSILELPCPH LSALASYYWS HGPAAVPEAS STVYNGSLLL481IVQDGVGGLY QCWATENGFS YPVISYWVDS QDQTLALDPE LAGIPREHVK VPLTRVSGGA541ALAAQQSYWP HFVTVTVLFA LVLSGALIIL VASPLRALRA RGKVQGCETL RPGEKAPLSR601EQHLQSPKEC RTSASDVDAD NNCLGTEVATRTRPLEQKLISEEDLAANDILDYKDDDDKV
629 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.