SEMA4A / Semaphorin 4A Antibody
Catalog Number: LS-C489151-100
100 µL / $309.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SEMA4A / Semaphorin 4A Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G91621-20
Catalog Number: LS-G91621
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SEMA4A (NM_001193302) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CORD10 | RP11-54H19.2 | RP35 | Sema B | Semaphorin-4A | Semaphorin-B | SEMB | SEMA4A | SEMAB
- Origin Species
- Human
- NCBI GenBank
- NM_022367
- SwissProt
- Q9H3S1
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIPWPASDRK KSECAFKKKS NEELQDSYLL PISEDKVMEG KGQSPFDPAH KHTAVLVDGM61LYSGTMNNFL GSEPILMRTL GSQPVLKTDN FLRWLHHDAS FVAAIPSTQV VYFFFEETAS121EFDFFERLHT SRVARVCKND VGGEKLLQKK WTTFLKAQLL CTQPGQLPFN VIRHAVLLPA181DSPTAPHIYA VFTSQWQVGG TRSSAVCAFS LLDIERVFKG KYKELNKETS RWTTYRGPET241NPRPGSCSVG PSSDKALTFM KDHFLMDEQV VGTPLLVKSG VEYTRLAVET AQGLDGHSHL301VMYLGTTTGS LHKAVVSGDS SAHLVEEIQL FPDPEPVRNL QLAPTQGAVF VGFSGGVWRV361PRANCSVYES CVDCVLARDP HCAWDPESRT CCLLSAPNLN SWKQDMERGN PEWACASGPM421SRSLRPQSRP QIIKEVLAVP NSILELPCPH LSALASYYWS HGPAAVPEAS STVYNGSLLL481IVQDGVGGLY QCWATENGFS YPVISYWVDS QDQTLALDPE LAGIPREHVK VPLTRVSGGA541ALAAQQSYWP HFVTVTVLFA LVLSGALIIL VASPLRALRA RGKVQGCETL RPGEKAPLSR601EQHLQSPKEC RTSASDVDAD NNCLGTEVATRTRPLEQKLISEEDLAANDILDYKDDDDKV
629 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
