Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RFWD2 / COP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122699-100
Catalog Number: LS-G122699
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COP1(RFWD2) (NM_001001740), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COP1 | HCOP1 | Putative ubiquitin ligase COP1 | RP11-318C24.3 | RFWD2 | RNF200 | RING finger protein 200
- Origin Species
- Human
- NCBI GenBank
- NM_022457
- SwissProt
- Q8NHY2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGSRQAGSG SAGTSPGSSA ASSVTSASSS LSSSPSPPSV AVSAAALVSG GVAQAAGSGG61LGGPVRPVLV APAVSGSGGG AVSTGLSRHS CAARPSAGVG GSSSSLGSGS RKRPLLAPLC121NGLINSYEDK SNDFVCPICF DMIEEAYMTK CGHSFCYKCI HQSLEDNNRC PKCNYVVDNI181DHLYPNFLVN ELILKQKQRF EEKRFKLDHS NGHRWQIFQD WLGTDQDNLD LANVNLMLEL241LVQKKKQLEA ESHAAQLQIL MEFLKVARRN KREEMSGLYS PVSEDSTVPQ FEAPSPSHSS301IIDSTEYSQP PGFSGSSQTK KQPWYNSTLA SRRKRLTAHF EDLEQCYFST RMSRISDDSR361TASQLDEFQE CLSKFTRYNS VRPLATLSYA SDLYNGSSIV SSIEFDRDCD YFAIAGVTKK421IKVYEYDTVI QDAVDIHYPE NEMTCNSKIS CISWSSYHKN LLASSDYEGT VILWDGFTGQ481RSKVYQEHEK RCWSVDFNLM DPKLLASGSD DAKVKLWSTN LDNSVASIEA KANVCCVKFS541PSSRYHLAFG CADHCVHYYD LRNTKQPIMV FKGHRKAVSY AKFVSGEEIV SASTDSQLKL601WNVGKPYCLR SFKGHINEKN FVGLASNGDY IACGSENNSL YLYYKGLSKT LLTFKFDTVK661SVLDKDRKED DTNEFVSAVC WRALPDGESN VLIAANSQGT IKVLELVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
707 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.