RFWD2 / COP1 Antibody
Catalog Number: LS-C824063-100
100 µg / $365.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RFWD2 / COP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G87141-20
Catalog Number: LS-G87141
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human COP1(RFWD2) (NM_022457) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COP1 | HCOP1 | Putative ubiquitin ligase COP1 | RP11-318C24.3 | RFWD2 | RNF200 | RING finger protein 200
- Origin Species
- Human
- NCBI GenBank
- NM_022457
- SwissProt
- Q8NHY2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGSRQAGSG SAGTSPGSSA ASSVTSASSS LSSSPSPPSV AVSAAALVSG GVAQAAGSGG61LGGPVRPVLV APAVSGSGGG AVSTGLSRHS CAARPSAGVG GSSSSLGSGS RKRPLLAPLC121NGLINSYEDK SNDFVCPICF DMIEEAYMTK CGHSFCYKCI HQSLEDNNRC PKCNYVVDNI181DHLYPNFLVN ELILKQKQRF EEKRFKLDHS VSSTNGHRWQ IFQDWLGTDQ DNLDLANVNL241MLELLVQKKK QLEAESHAAQ LQILMEFLKV ARRNKREQLE QIQKELSVLE EDIKRVEEMS301GLYSPVSEDS TVPQFEAPSP SHSSIIDSTE YSQPPGFSGS SQTKKQPWYN STLASRRKRL361TAHFEDLEQC YFSTRMSRIS DDSRTASQLD EFQECLSKFT RYNSVRPLAT LSYASDLYNG421SSIVSSIEFD RDCDYFAIAG VTKKIKVYEY DTVIQDAVDI HYPENEMTCN SKISCISWSS481YHKNLLASSD YEGTVILWDG FTGQRSKVYQ EHEKRCWSVD FNLMDPKLLA SGSDDAKVKL541WSTNLDNSVA SIEAKANVCC VKFSPSSRYH LAFGCADHCV HYYDLRNTKQ PIMVFKGHRK601AVSYAKFVSG EEIVSASTDS QLKLWNVGKP YCLRSFKGHI NEKNFVGLAS NGDYIACGSE661NNSLYLYYKG LSKTLLTFKF DTVKSVLDKD RKEDDTNEFV SAVCWRALPD GESNVLIAAN721SQGTIKVLEL VTRTRPLEQKLISEEDLAANDILDYKDDDDKV
731 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
