Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTPRA / RPTP-Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99910-100
Catalog Number: LS-G99910
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTP alpha(PTPRA) (NM_002836), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HLPR | Hptp alpha | HEPTP | HPTPA | PTPA | Ptpalpha | PTPRL2 | PTP alpha | PTPLCA-related phosphatase | R-PTP-alpha | RPTPA | Rptpalpha | Tyrosine phosphatase alpha | RPTP alpha | HPTPalpha | LRP | PTPase-alpha | PTPRA
- NCBI GenBank
- NM_002836
- SwissProt
- P18433
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 90.72 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSWFILVLL GSGLICVSAN NATTVAPSVG ITRLINSSTA EPVKEEAKTS NPTSSLTSLS61VAPTFSPNIT LGPTYLTTVN SSDSDNGTTR TASTNSIGIT ISPNGTWLPD NQFTDARTEP121WEGNSSTAAT TPETFPPSGN SDSKDRRDET PIIAVMVALS SLLVIVFIII VLYMLRFKKY181KQAGSHSNSF RLSNGRTEDV EPQSVPLLAR SPSTNRKYPP LPVDKLEEEI NRRMADDNKL241FREEFNALPA CPIQATCEAA SKEENKEKNR YVNILPYDHS RVHLTPVEGV PDSDYINASF301INGYQEKNKF IAAQGPKEET VNDFWRMIWE QNTATIVMVT NLKERKECKC AQYWPDQGCW361TYGNIRVSVE DVTVLVDYTV RKFCIQQVGD MTNRKPQRLI TQFHFTSWPD FGVPFTPIGM421LKFLKKVKAC NPQYAGAIVV HCSAGVGRTG TFVVIDAMLD MMHTERKVDV YGFVSRIRAQ481RCQMVQTDMQ YVFIYQALLE HYLYGDTELE VTSLETHLQK IYNKIPGTSN NGLEEEFKKL541TSIKIQNDKM RTGNLPANMK KNRVLQIIPY EFNRVIIPVK RGEENTDYVN ASFIDGYRQK601DSYIASQGPL LHTIEDFWRM IWEWKSCSIV MLTELEERGQ EKCAQYWPSD GLVSYGDITV661ELKKEEECES YTVRDLLVTN TRENKSRQIR QFHFHGWPEV GIPSDGKGMI SIIAAVQKQQ721QQSGNHPITV HCSAGAGRTG TFCALSTVLE RVKAEGILDV FQTVKSLRLQ RPHMVQTLEQ781YEFCYKVVQE YIDAFSDYAN FKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
802 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.