Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTPRA / RPTP-Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107715-100
Catalog Number: LS-G107715
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTP alpha(PTPRA) (NM_080840), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HLPR | Hptp alpha | HEPTP | HPTPA | PTPA | Ptpalpha | PTPRL2 | PTP alpha | PTPLCA-related phosphatase | R-PTP-alpha | RPTPA | Rptpalpha | Tyrosine phosphatase alpha | RPTP alpha | HPTPalpha | LRP | PTPase-alpha | PTPRA
- Origin Species
- Human
- NCBI GenBank
- NM_002836
- SwissProt
- P18433
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSWFILVLL GSGLICVSAN NATTVAPSVG ITRLINSSTA EPVKEEAKTS NPTSSLTSLS61VAPTFSPNIT LGPTYLTTVN SSDSDNGTTR TASTNSIGIT ISPNGTWLPD NQFTDARTEP121WEGNSSTAAT TPETFPPSDE TPIIAVMVAL SSLLVIVFII IVLYMLRFKK YKQAGSHSNS181FRLSNGRTED VEPQSVPLLA RSPSTNRKYP PLPVDKLEEE INRRMADDNK LFREEFNALP241ACPIQATCEA ASKEENKEKN RYVNILPYDH SRVHLTPVEG VPDSDYINAS FINGYQEKNK301FIAAQGPKEE TVNDFWRMIW EQNTATIVMV TNLKERKECK CAQYWPDQGC WTYGNIRVSV361EDVTVLVDYT VRKFCIQQVG DMTNRKPQRL ITQFHFTSWP DFGVPFTPIG MLKFLKKVKA421CNPQYAGAIV VHCSAGVGRT GTFVVIDAML DMMHTERKVD VYGFVSRIRA QRCQMVQTDM481QYVFIYQALL EHYLYGDTEL EVTSLETHLQ KIYNKIPGTS NNGLEEEFKK LTSIKIQNDK541MRTGNLPANM KKNRVLQIIP YEFNRVIIPV KRGEENTDYV NASFIDGYRQ KDSYIASQGP601LLHTIEDFWR MIWEWKSCSI VMLTELEERG QEKCAQYWPS DGLVSYGDIT VELKKEEECE661SYTVRDLLVT NTRENKSRQI RQFHFHGWPE VGIPSDGKGM ISIIAAVQKQ QQQSGNHPIT721VHCSAGAGRT GTFCALSTVL ERVKAEGILD VFQTVKSLRL QRPHMVQTLE QYEFCYKVVQ781EYIDAFSDYA NFKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
793 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.